SubtiBank SubtiBank
Version comparison:

2017-06-10 09:17:202025-04-08 11:11:14

description

Holliday junction DNA helicase, part of the RuvA-RuvB branch migration translocase

Holliday junction DNA helicase, part of the [[protein|RuvA]]-[[protein|RuvB]] branch migration translocase

locus

BSU27740

BSU_27740

geneLength

603

606

function

DNA repair/ recombination

[SW|DNA repair/ recombination]

ec

outlinks

bsu

BSU27740

BSU_27740

Gene

Coordinates

2,836,165 → 2,836,770

2,836,165 2,836,770

Gene

Phenotypes of a mutant

the inactivations of ''[[gene|recU]]'' and ''[[gene|ruvA]]-[[gene|ruvB]]'' are synthetically lethal [Pubmed|24770420]

the inactivations of ''[[gene|recU]]'' and ''[[gene|ruvA]]-[[gene|ruvB]]'' are synthetically lethal [Pubmed|24770420]

the inactivations of ''[[gene|recD2]]'' and ''[[gene|ruvA]]-[[gene|ruvB]]'' are synthetically lethal [pubmed|28527403]

The protein

Catalyzed reaction/ biological activity

inhibits resolution of Holliday junctions by [[protein|RecU]]-[[protein|RuvB]] [Pubmed|24770420]

inhibits resolution of Holliday junctions by [[protein|RecU]]-[[protein|RuvB]] [Pubmed|24770420]

ATP + H2O --> ADP + H+ + phosphate (according to UniProt)

The protein

Protein family

ruvA family (according to Swiss-Prot)

ruvA family (single member, according to UniProt)

Biological materials

Mutant

1A896 (no resistance), [Pubmed|16020779], available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1A896&Search=1A896 BGSC]

GP3530 (Δ([[gene|ruvA]]-[[gene|ruvB]])::kan), available in [SW|Jörg Stülke]'s lab

1A896 (no resistance), [Pubmed|16020779], available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1A896&Search=1A896 BGSC]

BKE27740 ([[gene|ruvA]]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE27740 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CACGCAAATTCACCTCTTTG, downstream forward: _UP4_AAACTATTAAAGTAAGGTGA

BKK27740 ([[gene|ruvA]]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK27740 BGSC] and in [SW|Jörg Stülke]'s lab, [Pubmed|28189581], upstream reverse: _UP1_CACGCAAATTCACCTCTTTG, downstream forward: _UP4_AAACTATTAAAGTAAGGTGA

References

Original publications

16267290, 24770420, 16020779

16267290, 24770420, 16020779, 9628481, 28527403

The protein

Paralogous protein(s)

[[this]]

The protein

Structure

[PDB|1BDX] (from E. coli, 34% identity) [pubmed|9628481]