2017-06-10 09:17:202025-04-08 11:11:14
description
Holliday junction DNA helicase, part of the RuvA-RuvB branch migration translocase
Holliday junction DNA helicase, part of the [[protein|RuvA]]-[[protein|RuvB]] branch migration translocase
locus
BSU27740
BSU_27740
geneLength
603
606
function
DNA repair/ recombination
[SW|DNA repair/ recombination]
ec
outlinks
bsu
BSU27740
BSU_27740
Gene
Coordinates
2,836,165 → 2,836,770
2,836,165 2,836,770
Gene
Phenotypes of a mutant
the inactivations of ''[[gene|recU]]'' and ''[[gene|ruvA]]-[[gene|ruvB]]'' are synthetically lethal [Pubmed|24770420]
the inactivations of ''[[gene|recU]]'' and ''[[gene|ruvA]]-[[gene|ruvB]]'' are synthetically lethal [Pubmed|24770420]
the inactivations of ''[[gene|recD2]]'' and ''[[gene|ruvA]]-[[gene|ruvB]]'' are synthetically lethal [pubmed|28527403]
The protein
Catalyzed reaction/ biological activity
inhibits resolution of Holliday junctions by [[protein|RecU]]-[[protein|RuvB]] [Pubmed|24770420]
inhibits resolution of Holliday junctions by [[protein|RecU]]-[[protein|RuvB]] [Pubmed|24770420]
ATP + H2O --> ADP + H+ + phosphate (according to UniProt)
The protein
Protein family
ruvA family (according to Swiss-Prot)
ruvA family (single member, according to UniProt)
Biological materials
Mutant
1A896 (no resistance), [Pubmed|16020779], available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1A896&Search=1A896 BGSC]
GP3530 (Δ([[gene|ruvA]]-[[gene|ruvB]])::kan), available in [SW|Jörg Stülke]'s lab
1A896 (no resistance), [Pubmed|16020779], available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1A896&Search=1A896 BGSC]
BKE27740 ([[gene|ruvA]]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE27740 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CACGCAAATTCACCTCTTTG, downstream forward: _UP4_AAACTATTAAAGTAAGGTGA
BKK27740 ([[gene|ruvA]]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK27740 BGSC] and in [SW|Jörg Stülke]'s lab, [Pubmed|28189581], upstream reverse: _UP1_CACGCAAATTCACCTCTTTG, downstream forward: _UP4_AAACTATTAAAGTAAGGTGA
References
Original publications
The protein
Paralogous protein(s)
[[this]]
The protein
Structure
[PDB|1BDX] (from E. coli, 34% identity) [pubmed|9628481]